SCM

Presentation

The microscopy facility of the Centre Universitaire des Saints-Pères (CUSP) offers access to photonic microscopy techniques (widefield, confocal, Airyscan, slide scanner) along with tools for image analysis and storage solutions. The facility is run by an operations manager and a scientific manager. The main decisions are made with the committee, representing each CUSP research unit and which is consulted approximately once a month. Located on the 4th floor of the Faculté des Sciences Fondamentales et Biomédicales, the facility is open to the researchers, engineers, technicians and students of the building and to external teams/private companies. The ambition of the facility is to expand according to the users’ needs.

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Team

Documentation

Charte

Equipements

Laboratory fume cupboard – Sodatec

– Room H420A

  • 3 micropipets Gilson (P20, P200, P2000) + pipet tips
  • Dirt containers (sharp objetcts/paraformaldehyde)
  • Each user has to bring his equipment and take it back after each session.
  • Training and Booking are mandatory
  • Free of charge

ZEISS LSM880 Fast Airyscan

– Room H423B

  • High-resolution confocal
  • 3D acquisition, time lapse, tiles, multi-positions, FRAP, spectral deconvolution
  • Fixed/living samples
  • Temperature and CO2 control
  • Definite focus 2
  • Software ZEN Black 2.3
Lasers Objectives Detection
405 nm
445 nm
488 nm
514 nm
561 nm
594 nm
633 nm
10x/0.45 Dry WD : 2mm
25x/0.8 DIC Multi-immersion WD : 0.57 mm
40x/1.2 Water Autocorr. WD : 0.28 mm
63x/1.4 Oil DIC WD : 0.19 mm
2 PMTs
1 spectral PMT GaAsP
1 Airyscan detector GaAsP
1 Trans-PMT Software ZEN Black 2.3

 

ZEISS LSM710 Confocal

– Room H423A

  • 3D acquisition, time lapse, tiles, multi-positions, FRAP, spectral deconvolution
  • Fixed/living samples
  • Software ZEN Black 2.1

 

Lasers Objectives Detection
405 nm
445 nm
488 nm
514 nm
561 nm
633 nm
10x/0.3 Dry WD : 5.2 mm
20x/0.8 Dry WD : 0.55 mm
40x/1 Water DIC WD : 2.5 mm
63x/1.4 Oil WD : 0.19 mm
63x/1.2 Water WD : 0.28 mm
2 PMTs
1 spectral PMT
1 Trans-PMT

 

ZEISS LSM510 Confocal

– Room H423A

  • 3D acquisition, time lapse
  • Fixed samples
  • Software ZEN 2009

 

Lasers Objectives Detection
Argon 458/477/488/514 nm ; 30 mW
543 nm 1 mW
633 nm 5 mW
2.5x/0.12 Dry WD : 6.3 mm
10x/0.3 Dry WD : 5.2 mm
20x/0.5 Dry WD : 2 mm
40x/1.3 Oil DIC WD : 0.2 mm
63x/1.4 Oil DIC WD : 0.19 mm
2 PMTs
1 Trans-PMT

 

ZEISS AxioScan.Z1 Slide scanner

– Room H423A

  • 100 slides magazine, 3D acquisitions
  • Fixed samples
  • Brightfield, Fluorescence, Polarization
  • Possible correlation with LSM 880 Airyscan
  • Software : ZEN 2.6
LEDs Objectives Detection
385 nm
430 nm
475 nm
511 nm
555 nm
590 nm
630 nm
Plan Apochromat 10x, 20x, 40x Dry Brightfield : Hitachi HV F202
Fluo : Hamamatsu ORCA-Flash 4.0

 

 

Nikon Eclipse TE-2000E Widefield microscope

– Room H438A

  • 3D acquisition, time lapse, tiles, mutli-positions
  • Brightfield/Fluorescence
  • Fixed/living samples
  • Temperature control
  • Fluorescent lamp metal halide Nikon Intensilight 130W
  • Software : NIS-Elements AR 3
Objectives Detection
4x/0.13 Dry WD : 17.2 mm
10x/0.30 Dry WD : 16 mm
20x/0.75 Dry DIC WD : 1 mm
40x/1.30 Oil DIC WD : 0.2 mm
100x/1.45 Oil WD : 0.13 mm
Photometrics CoolSnap HQ2 (monochromatic)
QImaging Color Retiga 2000R (Color)

 

ZEISS Axioscope.A1 Widefield microscope

– Room H438A

  • Brightfield/Fluorescence
  • Fixed samples
  • Software : ProgRes Capture Pro
Objectives Detection
5x/0.16 Dry WD : 18.5 mm
10x/0.30 Dry WD : 5.2 mm
40x/1.3 Oil WD : 0.21 mm
40x/1.30 Oil DIC WD : 0.2 mm
100x/1.4 Oil WD : 0.17 mm
Progres MF-USB Jenoptik

 

Analysis Workstation 1

– Room H420B

  • AutoQuant x3 Deconvolution software – Free of charge

Analysis Workstation 2

– Room H420B

  • Imaris 9.1.2 (Cell, Imaris XT, Coloc, Filament Tracer, Vantage, Batch)
  • ZEN Blue lite
  • ZEN Black lite (with Airscan processing module)
  • NIS Elements AR Off-line
  • ImageJ/Fiji

STORAGE SOLUTIONS AND POLICY

The images acquired on our systems are first stored on the acquisition computer. After each session on the systems, the user can transfer the files from the computer to one of our two servers (SCMRAID : 12 To or SERVEUR880 : 100 To). The images are stored on our computers for 6 months and for 2 years on our servers. After the delay, they are automatically deleted.

PRESTATIONS

The microscopy facility provides trainings on all the equipments mentioned above, under request. The autonomous users can ask to be assisted by a member of the facility if needed. We also provide our expertise and advices to guide our users in their experiments regarding sample preparation, if possible, imaging and image analysis.

PRICES

Equipements Tarifs d’utilisation interne académique CUSP (HT) Tarifs d’utilisation externe académique CUSP (HT) Tarifs d’utilisation privée (HT)
Sorbonne (Hotte Laboratoire) Gratuit Gratuit Gratuit
Slide Scanner Zeiss AxioScan.Z1 Scans < 8heures (mardi et jeudi) : 5€/heure Scans < 8heures (mardi et jeudi) : 7€/heure Scans < 8heures (mardi et jeudi) : 10€/heure
Scans longs journée (9h – 17h) : 25€ Scans longs journée (9h – 17h) : 32€ Scans longs journée (9h – 17h) : 50€
Scans longs nuit (17h-9h) : 40€ Scans longs nuit (17h-9h) : 50€ Scans longs nuit (17h-9h) : 80€
Microscope WF 1 – NIKON Eclipse-TE2000E 10 € / Heure 12 € / Heure 20 € / Heure
Microscope WF 2 – ZEISS Axioscope A1 Gratuit Gratuit Gratuit
Microscope Confocal 1 – ZEISS LSM 510 18 € / Heure 22,5 € / Heure 36 € / Heure
Microscope Confocal 2 – ZEISS LSM 710 20 € / Heure 25 € / Heure 40 € / Heure
Microscope Confocal 3 – ZEISS LSM 880 25 € / Heure 41 € / Heure 70 € / Heure
Analysis WS 1 – Déconvolution Gratuit 5 € / Heure 10 € / Heure
Analysis WS 2 – Reconstruction 3D Gratuit en autonomie Gratuit en autonomie 5€/h en autonomie
Prestation Accompagnement analyse : 5€/h Prestation Accompagnement analyse : 10€/h Prestation Accompagnement analyse : 30€/h
EquipementsSorbonne (Hotte Laboratoire)
Tarifs 1Gratuit
Tarifs 2Gratuit
Tarifs 3Gratuit
Equipements Slide Scanner Zeiss AxioScan.Z1
Tarif interne académique (HT) Scans < 8heures (mardi et jeudi) : 5€/heure
Scans longs journée (9h – 17h) : 25€
Scans longs nuit (17h-9h) : 40€
Tarif interne académique (HT) Scans < 8heures (mardi et jeudi) : 7€/heure
Scans longs journée (9h – 17h) : 32€
Scans longs nuit (17h-9h) : 50€
Tarif 3 Scans < 8heures (mardi et jeudi) : 10€/heure
Scans longs journée (9h – 17h) : 50€
Scans longs nuit (17h-9h) : 80€
EquipementsMicroscope WF 1 – NIKON Eclipse-TE2000E
Tarifs 110 € / Heure
Tarifs 212 € / Heure
Tarifs 320 € / Heure
EquipementsMicroscope WF 2 – ZEISS Axioscope A1
Tarifs 1Gratuit
Tarifs 2Gratuit
Tarifs 3Gratuit

Contact / Access / Booking

Any question ? Please contact this address in order to have a better answser : scm@u-paris-sciences.fr / 01 76 53 10 77

Find the Microscopy Platform on the 4th floor of the Saints-Pères building and all the access details by following this link.

Gallery