SCM

Presentation

The microscopy facility of the Campus Saint Germain des Prés (CSGP) offers access to photonic microscopy techniques (widefield, confocal, Airyscan, slide scanner) along with tools for image analysis and storage solutions. The facility is run by an operations manager and a scientific manager. The main decisions are made with the committee, representing each CSGP research unit and which is consulted approximately once a month. Located on the 4th floor of the UFR des Sciences Fondamentales et Biomédicales, the facility is open to the researchers, engineers, technicians and students of the building and to external teams/private companies. The ambition of the facility is to expand according to the users’ needs.

News

Atelier QuPath
Afin d'inaugurer le nouveau format d'atelier de la plateforme (SCMtech) nous avons le plaisir d'inviter Perrine Frère qui viendra nous présenter le logiciel d'analyse d'image QuPath. L'Atelier QuPath aura lieu le Jeudi 29 Février 2024 à 14h en P436 14h-14h30 :...
Leica : THUNDER Imaging Systems
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IMARIS 3D/4D Visualization & Analysis Software
An introduction to 3D visualization & analysis techniques in Imaris by Georgia Golfis, PhD 16 February 2023Room P436/Salle du Conseil 14h00-1630 © Tous droits réservés 14:00 - 15:00 An introduction to 3D visualization & analysis techniques in Imaris P436 15:00...
Meet the UltraMicroscope Blaze TM
Discover how the UltraMicroscope Blaze TM deep learning revolutionized Brainimaging and Analysis Talk Given by Christian Feuillet - Miltenyi BiotecImage d’une lame virtuelle de moelle osseuse. © Tous droits réservés Contact & information :Fabrice...
arivis Vision4D – Introduction
arivis Vi sion4D - 15th Sep. 11-16H - UFR des Sciences Fondamentaleset BiomédicalesImage d’une lame virtuelle de moelle osseuse. © Tous droits réservés Arivis provides an image analysis platform with big data and ease of use in mind. Our tools are designed to take...

Team

 Equipements

ZEISS LSM880 Fast Airyscan

– Room H423B

  • High-resolution confocal
  • 3D acquisition, time lapse, tiles, multi-positions, FRAP, spectral deconvolution
  • Fixed/living samples
  • Temperature and CO2 control
  • Definite focus 2
  • Software ZEN Black 2.3
Lasers Objectives Detection
405 nm
445 nm
488 nm
514 nm
561 nm
594 nm
633 nm
10x/0.45 Dry WD : 2mm
40x/1.2 Water Autocorr. WD : 0.28 mm
63x/1.4 Oil DIC WD : 0.19 mm
2 PMTs
1 spectral PMT GaAsP
1 Airyscan detector GaAsP
1 Trans-PMT

 

ZEISS LSM710 Confocal

– Room H423A

  • 3D acquisition, time lapse, tiles, multi-positions, FRAP, spectral deconvolution
  • Fixed/living samples
  • Software ZEN Black 2.1

 

Lasers Objectives Detection
405 nm
458 nm
488 nm
514 nm
561 nm
633 nm
10x/0.3 Dry WD : 5.2 mm
20x/0.8 Dry WD : 0.55 mm
40x/1 Water DIC WD : 2.5 mm
63x/1.4 Oil WD : 0.19 mm
63x/1.2 Water WD : 0.28 mm
2 PMTs
1 spectral PMT
1 Trans-PMT

 

ZEISS LSM510 Confocal

– Room H423A

  • 3D acquisition, time lapse
  • Fixed samples
  • Software ZEN 2009

 

Lasers Objectives Detection
458 nm
477 nm
488 nm
514 nm
543 nm
633 nm
2.5x/0.12 Dry WD : 6.3 mm
10x/0.3 Dry WD : 5.2 mm
20x/0.5 Dry WD : 2 mm
40x/1.3 Oil DIC WD : 0.2 mm
63x/1.4 Oil DIC WD : 0.19 mm
2 PMTs
1 Trans-PMT

 

ZEISS AxioScan.Z1 Slide scanner

– Room H423A

  • 100 slides magazine, 3D acquisitions
  • Fixed samples
  • Brightfield, Fluorescence, Polarization
  • Graphic tab
  • Software : ZEN 3.1
LEDs Objectives Detection
385 nm
430 nm
475 nm
511 nm
555 nm
590 nm
630 nm

10x/0.45 Dry Plan Apochromat

20x/0.80 Dry Plan Apochromat

40x/0.95 Dry Plan Apochromat

Hamamatsu ORCA-Flash 4.0 (monochromatic)

Hitachi HV F202 (color)

 

 

Nikon Eclipse TE-2000E Widefield microscope

– Room H438A

  • 3D acquisition, time lapse, tiles, mutli-positions
  • Brightfield/Fluorescence
  • Fixed/living samples
  • Temperature control
  • Fluorescent lamp metal halide Nikon Intensilight 130W
  • Software : NIS-Elements AR 3
Objectives Detection
4x/0.13 Dry WD : 17.2 mm
10x/0.30 Dry WD : 16 mm
20x/0.75 Dry DIC WD : 1 mm
40x/1.30 Oil DIC WD : 0.2 mm
100x/1.45 Oil WD : 0.13 mm
Photometrics CoolSnap HQ2 (monochromatic)
QImaging Color Retiga 2000R (Color)

 

ZEISS Axioscope.A1 Widefield microscope

– Room H438A

  • Brightfield/Fluorescence
  • Fixed samples
  • Software : ProgRes Capture Pro
Objectives Detection
5x/0.16 Dry WD : 18.5 mm
10x/0.30 Dry WD : 5.2 mm
100x/1.4 Oil WD : 0.17 mm
(sur demande 20x, 40x)
Progres MF-USB Jenoptik (monochromatic)

 

Analysis Workstation 1

– Room H420B

  • AutoQuant X 3.1.3 Deconvolution software
  • Ilastik 1.4.0
  • QuPath v0.5.0

Analysis Workstation 2

– Room H420B

  • Imaris 10 (Cell, Imaris XT, Coloc, Filament Tracer, Vantage, Batch)
  • ZEN Blue lite
  • ZEN Black lite (with Airscan processing module)
  • NIS Elements AR Off-line
  • ImageJ/Fiji

Laboratory fume cupboard – Sodatec

– Room H420A

  • 3 micropipets Gilson (P20, P200, P2000) + pipet tips
  • Dirt containers (sharp objetcts/paraformaldehyde)
  • Precision Balance

STORAGE SOLUTIONS AND POLICY

The images acquired on our systems are first stored on the acquisition computer. After each session on the systems, the user can transfer the files from the computer to our server (100 To). The images are stored on our computers for 6 months and for 3 years on our servers. After the delay, they are automatically deleted.

PRESTATIONS

The microscopy facility provides trainings on all the equipments mentioned above, under request. The autonomous users can ask to be assisted by a member of the facility if needed. We also provide our expertise and advices to guide our users in their experiments regarding sample preparation, if possible, imaging and image analysis.

PRICES

2023

Equipements Tarifs d'utilisation interne académique CSG (HT) Tarifs d'utilisation externe académique Paris Cité (HT) Tarifs d'utilisation externe académique (HT) Tarifs d'utilisation privée (HT)
Sorbonne (Hotte Laboratoire) Gratuit Gratuit Gratuit Gratuit
Microscope Zeiss Primo Star Gratuit Gratuit Gratuit Gratuit
Slide Scanner Zeiss AxioScan.Z1 6 € / Heure
Scans longs nuit
(17h-9h) : 40€
8 € / Heure
Scans longs nuit
(17h-9h) : 55€
10 € / Heure
Scans longs nuit
(17h-9h) : 80€
21 € / Heure
Scans longs nuit
(17h-9h) : 160€
Microscope WF 1 - NIKON Eclipse-TE2000E 9 € / Heure 9 € / Heure 12 € / Heure 20 € / Heure
Microscope WF 2 - ZEISS Axioscope A1 Gratuit Gratuit Gratuit Gratuit
Microscope Confocal 1 - ZEISS LSM 510 18 € / Heure 20 € / Heure 22 € / Heure 36 € / Heure
Microscope Confocal 2 - ZEISS LSM 710 21 € / Heure 23 € / Heure 26 € / Heure 42 € / Heure
Microscope Confocal 3 - ZEISS LSM 880 26 € / Heure 32 € / Heure 41 € / Heure 73 € / Heure
Analysis WS 1 - Déconvolution Gratuit Gratuit 5 € / Heure 10 € / Heure
Analysis WS 2 – Reconstruction 3D Gratuit Gratuit 7 € / Heure 15 € / Heure

Contact / Access / Documention

Any question ? Please contact this address in order to have a better answser : scm@u-paris-sciences.fr / 01 76 53 10 77

Find the Microscopy Platform on the 4th floor of the Saints-Pères building and all the access details by following this link.

Charte SCM (PDF)
Remerciements UAR (PDF)

Partners

Publications

2023
  • KCa1.1 channels contribute to optogenetically driven post-stimulation silencing in cerebellar molecular layer interneurons.
    Kassa M, Bradley J, Jalil A, Llano I
    J Gen Physiol. 2023 Jan 2;155(1):e202113004.doi: 10.1085/jgp.202113004. Epub 2022 Nov 3.
    PMID: 36326690
  • Virus-Free Method to Control and Enhance Extracellular Vesicle Cargo Loading and Delivery
    Bui S, Dancourt J, Lavieu G
    Bio Mater. 2023 Mar 20;6(3):1081-1091. doi: 10.1021/acsabm.2c00955. Epub 2023 Feb 13.
    PMID: 36781171
2022
  • A link between agrin signalling and Cav3.2 at the neuromuscular junction in spinal muscular atrophy
    Perrine Delers, Delphine Sapaly, Badih Salman, Stephan De Waard, Michel De Waard & Suzie Lefebvre
    Sci Rep . 2022 Nov 8;12(1):18960.doi: 10.1038/s41598-022-23703-x
    PMID: 36347955
  • Label-free, fast, 2-photon volume imaging of the structural organization of peripheral neurons and glia in the enteric ganglia
    Doriane Hazart, 1,2,#,$ Brigitte Delhomme, 1 Martin Oheim 1,*,† and Clément Ricard 1
    Front Neuroanat. 2023 Feb 9;16:1070062.doi: 10.3389/fnana.2022.1070062. eCollection 2022.
    PMID: 36844894
  • The cell polarity protein Vangl2 in the muscle shapes the neuromuscular synapse by binding to and regulating the tyrosine kinase MuSK
    Myriam Boëx, Steve Cottin, Marius Halliez, Stéphanie Bauché, Céline Buon, Nathalie Sans, Mireille Montcouquiol, Jordi Molgó, Muriel Amar, Arnaud Ferry, Mégane Lemaitre, Andrée Rouche, Dominique Langui, Asha Baskaran, Bertrand Fontaine, Julien Messéant, Laure Strochlic
    Sci Signal. 2022 May 17;15(734):eabg4982. Epub 2022 May 17.
    PMID: 35580169
2021
2020
2019

Gallery