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NMR for structural and metabolomics studies

Actu :

RMN – Metabo Paris Santé

RMN – Metabo Paris Santé

L’Université de Paris se dote du premier système national d’analyse métabolique à haut débit des fluides biologiques chez l’Homme par résonance magnétique Dès octobre 2019, l’Université de Paris et sa plateforme MétaboParis-Santé, sera doté du tout nouveau système,...

The Nuclear Magnetic Resonance (NMR) platform deals with requests for chemical and / or biological analyzes from the private and public sectors, mainly for or structural studies or metabolomics studies.

This platform, which relies on the spectroscopic expertise developed in the « NMR of Biological Substances of Interest » team (Dir N. Giraud) within the UMR 8601, allows to implement at the same time Routine 1D and multi-dimensional NMR analyzes, but also specifically develop complex sample analysis strategies in the context of scientific collaborations. Our equipment, our protocols, and the training of our staff allow us to collaborate with academic, industrial, and / or medical laboratories.

-Structural analysis:

The platform has equipment to carry out the main so-called routine analyzes for the structural characterization of your products. The measurements are performed on a 500 MHz NMR spectrometer (Fig. 1) equipped with a sensitivity-optimized measurement head for 1D and 2D experiments involving 1H, 13C detection (cryoprobe cooled with liquid helium). Analyzes of the 19F or 31P cores are also possible on another device.

Figure 1: NMR spectrometer with cryoprobe 13C, 1H and automation.

-Metabolomic analysis (MetaboParis-Santé project):
Since 2012, the platform has developed an original metabolomic activity by NMR using non-targeted methods (Fig. 2), applied to the field of health. In this field, which has undergone a major change over the last fifteen years, we are able to offer a global vision of the metabolites produced by the human body or cells. We can help advance the understanding of the biological mechanisms of several diseases, or even their treatment, based on this type of approach. We have a panel of expertise covering all the crucial steps for the validation of an efficient analysis method at the interface of chemistry and biochemistry: sample preparation, spectroscopic development, development of an analytical method (targeted or untargeted), biological interpretation. In this context, we benefit from a network of scientific collaborations, notably in AP-HP hospitals, which has enabled us to develop a national « MetaboParis-Santé » project focused on the health sciences.

In October 2019, this platform will have a unique equipment in France, meeting international standards and a high reproducibility, using a targeted method (Fig. 2) for large scale studies of biofluids (around 100 samples / day).

 

Figure 2: Metabolomics methods by NMR

The team

Actu :

RMN – Metabo Paris Santé

L’Université de Paris se dote du premier système national d’analyse métabolique à haut débit des fluides biologiques chez l’Homme par résonance magnétique Dès octobre 2019, l’Université de Paris et sa plateforme MétaboParis-Santé, sera doté du tout nouveau système,...